XAF1 Rabbit Polyclonal Antibody

CAT#: TA306193

Rabbit Polyclonal XAF-1 Antibody

Product Images

Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody (IN) at 1 (lane A), 2 (lane B), and 4 (lane C) ug/ml, respectively.

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  Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: XIAP associated factor 1
• Database Link:
  NP_059993
  Entrez Gene 54739 Human
  UniProt ID Q6GPH4
• Background: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and –9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).
• Synonyms: BIRC4BP; HSXIAPAF1; XIAPAF1