MDA5 (IFIH1) Rabbit Polyclonal Antibody

CAT#: TA306393

Rabbit Polyclonal MDA5 Antibody

Product Images

Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

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  Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 ug/ml.

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  Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: MDA5 antibody was raised against a 16 amino acid peptide from near the center of human MDA5.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interferon induced with helicase C domain 1
• Database Link:
  NP_071451
  Entrez Gene 71586 MouseEntrez Gene 64135 Human
  UniProt ID Q9BYX4
• Background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
• Synonyms: AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2

Reference Data

• Protein Pathways: RIG-I-like receptor signaling pathway