ORAI3 Rabbit Polyclonal Antibody

CAT#: TA306420

Rabbit Polyclonal ORAI3 Antibody

Product Images

Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.

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  Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ORAI3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human ORAI3.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ORAI calcium release-activated calcium modulator 3
• Database Link:
  NP_689501
  Entrez Gene 269999 MouseEntrez Gene 309000 RatEntrez Gene 93129 Human
  UniProt ID Q9BRQ5
• Background: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
• Synonyms: TMEM142C

Reference Data

• Protein Families: Transmembrane