AKR1C1 Rabbit Polyclonal Antibody

CAT#: TA308032

Rabbit Polyclonal antibody to AKR1C1 (aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase))

Product Images

Sample (30 ug whole cell lysate). A:Raji. 10% SDS PAGE. TA308032 diluted at 1:1000

• 

  AKR1C1 antibody detects AKR1C1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 10 % SDS-PAGE. AKR1C1 antibody (TA308032) dilution: 1:1000

• 

  Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using AKR1C1 (TA308032) antibody at 1:500 dilution.

• 

  Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 (TA308032) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 22 and 248 of AKR1C1 (Uniprot ID#Q04828)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37 kDa
• Gene Name: aldo-keto reductase family 1, member C1
• Database Link:
  NP_001344
  Entrez Gene 1645 Human
  UniProt ID Q04828
• Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]
• Synonyms: 2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DD2; DDH; DDH1; H-37; HAKRC; HBAB; MBAB

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450