PCBP2 Rabbit Polyclonal Antibody

CAT#: TA308051

Rabbit Polyclonal antibody to PCBP2 (poly(rC) binding protein 2)

Product Images

Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308051 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. TA308051 diluted at 1:1000.

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  PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PCBP2 antibody [N1C1] (TA308051) dilution: 1:500.

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  PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PCBP2 antibody [N1C1] (TA308051) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed A431, using PCBP2 (TA308051) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 193 of PCBP2 (Uniprot ID#Q15366)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: poly(rC) binding protein 2
• Database Link:
  NP_005007
  Entrez Gene 18521 MouseEntrez Gene 363005 RatEntrez Gene 5094 Human
  UniProt ID Q15366
• Background: The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: hnRNP-E2; HNRNPE2; HNRPE2
• Note: Seq homology of immunogen across species: Rat (100%), Pig (100%), Bovine (100%)