alpha Tubulin (TUBA4A) Rabbit Polyclonal Antibody

CAT#: TA308420

Rabbit polyclonal anti-TUBA4A(alpha Tubulin) antibody, Loading control

Product Images

Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: HeLa. D: Molt-4. 10% SDS PAGE. TA308420 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA308420 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded H661 xenograft, using alpha Tubulin 4a (TA308420) antibody at 1:100 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using alpha Tubulin 4a (TA308420) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:3000, IHC 1:100-1:1000, IF 1:100 - 1:1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 21 and 243 of alpha Tubulin 4a (Uniprot ID#P68366)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: tubulin alpha 4a
• Database Link:
  NP_005991
  Entrez Gene 22145 MouseEntrez Gene 316531 RatEntrez Gene 7277 Human
  UniProt ID P68366
• Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq]
• Synonyms: ALS22; H2-ALPHA; TUBA1
• Note: Seq homology of immunogen across species: Rat (100%), Sheep (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection