Nkx2.5 (NKX2-5) Rabbit Polyclonal Antibody

CAT#: TA308654

Rabbit Polyclonal antibody to Nkx2.5 (NK2 transcription factor related, locus 5 (Drosophila))

Product Images

Sample (30 ug whole cell lysate). A:H1299. B:Raji. 10% SDS PAGE. TA308654 diluted at 1:1000

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  Sample (50 ug of whole cell lysate). A: mouse heart. 10% SDS PAGE. TA308654 diluted at 1:1000.

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  Nkx2.5 antibody detects NKX2-5 protein by Western blot analysis. A. 50 ug rat heart lysate/extract. 10 % SDS-PAGE. Nkx2.5 antibody (TA308654) dilution: 1:500

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  Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5 (TA308654) antibody at 1:200 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NKX2-5 (TA308654) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 218 of Nkx2.5 (Uniprot ID#P52952)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35 kDa
• Gene Name: NK2 homeobox 5
• Database Link:
  NP_004378
  Entrez Gene 18091 MouseEntrez Gene 1482 Human
  UniProt ID P52952
• Background: Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation. [supplied by OMIM]
• Synonyms: CHNG5; CSX; CSX1; HLHS2; NKX2.5; NKX2E; NKX4-1; VSD3

Reference Data

• Protein Families: Transcription Factors