KPNA2 Rabbit Polyclonal Antibody

CAT#: TA308727

Rabbit polyclonal antibody to karyopherin alpha 2 (karyopherin alpha 2 (RAG cohort 1, importin alpha 1))

Product Images

Sample (30 ug of whole cell lysate). A:HeLa S3. B:Hep G2. C:MOLT4. 10% SDS PAGE. TA308727 diluted at 1:500

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  karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: karyopherin alpha 2 protein stained by karyopherin alpha

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 468 and 529 of KPNA2 (Uniprot ID#P52292)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58 kDa
• Gene Name: karyopherin subunit alpha 2
• Database Link:
  NP_002257
  Entrez Gene 16647 MouseEntrez Gene 85245 RatEntrez Gene 3838 Human
  UniProt ID P52292
• Background: The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]
• Synonyms: IPOA1; QIP2; RCH1; SRP1-alpha; SRP1alpha
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Rhesus Monkey (92%), Chimpanzee (100%)

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency