RACGAP1 Rabbit Polyclonal Antibody

CAT#: TA308788

Rabbit Polyclonal antibody to RACGAP1 (Rac GTPase activating protein 1)

Product Images

Sample (50 ug of whole cell lysate). A: mouse heart. 7.5% SDS PAGE. TA308788 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. B: Raji. 7.5% SDS PAGE. TA308788 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RACGAP1 (TA308788) antibody at 1:500 dilution.

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  Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1 (TA308788) antibody at 1:500 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1 (TA308788) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 202 of RACGAP1 (Uniprot ID#Q9H0H5)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: Rac GTPase activating protein 1
• Database Link:
  NP_001119576
  Entrez Gene 26934 MouseEntrez Gene 315298 RatEntrez Gene 29127 Human
  UniProt ID Q9H0H5
• Background: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases. [supplied by OMIM]
• Synonyms: CYK4; HsCYK-4; ID-GAP; MgcRacGAP
• Note: Seq homology of immunogen across species: Rat (84%)

Reference Data

• Protein Families: Druggable Genome