HPRT (HPRT1) Rabbit Polyclonal Antibody

CAT#: TA308902

Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)

Product Images

Sample (30 ug of whole cell lysate). A: Molt-4. B: Raji. 12% SDS PAGE. TA308902 diluted at 1:1000.

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  HPRT antibody detects HPRT1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 12 % SDS-PAGE. HPRT antibody (TA308902) dilution: 1:500

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  Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HPRT (TA308902) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT (TA308902) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: hypoxanthine phosphoribosyltransferase 1
• Database Link:
  NP_000185
  Entrez Gene 24465 RatEntrez Gene 442945 DogEntrez Gene 3251 Human
  UniProt ID P00492
• Background: HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]). [supplied by OMIM]
• Synonyms: HGPRT; HPRT
• Note: Seq homology of immunogen across species: Chicken (94%), Dog (96%), Pig (97%), Rabbit (98%), Rat (96%), Xenopus laevis (92%), Zebrafish (93%), Bovine (94%), Xenopus tropicalis (92%)

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism