MAP3K8 Rabbit Polyclonal Antibody

CAT#: TA312191

Rabbit polyclonal COT (Ab-290) antibody

Product Images

Western blot analysis of extracts from 293 cells treated with LPS (100ng/ml, 30min), using COT (Ab-290) antibody (#TA312191, Line 1 and 2).The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using COT (Ab-290) antibody (#TA312191).The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HuvEc cells, using COT (Ab-290) antibody (#TA312191).The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:300~1000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human COT around the phosphorylation site of threonine 290 (R-G-TP-E-I).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase kinase kinase 8
• Database Link:
  NP_005195
  Entrez Gene 26410 MouseEntrez Gene 116596 RatEntrez Gene 1326 Human
  UniProt ID P41279
• Synonyms: AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2
• Note: COT (Ab-290) antibody detects endogenous levels of total COT protein.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway