Pogz Rabbit Polyclonal Antibody

CAT#: TA319377

Rabbit polyclonal anti-Pogz antibody

Product Images

Western blot using affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000. Personal Communication, K.O. Gudmundsson and J. Keller, NCI, Frederick, MD.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:100,000 to 1:400,000, WB: 1:2,000 to 1:20,000
• Reactivities: Human, Dog, Short-tailed Opossum, Bovine, Rat, Chimpanzee, Macaque, Olive Baboon, Chicken
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: pogo transposable element with ZNF domain
• Database Link:
  NP_766271
  Entrez Gene 23126 HumanEntrez Gene 310658 RatEntrez Gene 100856450 Dog
  UniProt ID Q8BZH4
• Synonyms: KIAA0461; MGC71543; OTTHUMP00000014469; SUHW5; ZNF280E; ZNF635; ZNF635m
• Note: This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Pogz is a zinc-finger protein containing at least 8 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. Members of this family of integrases share structural homologies and show similar catalytic activity to the RAG1 and RAG2 proteins that initiate V(D)J recombination in lymphoid progenitors. Mouse Pogz has a human homolog with a base pair similarity of 90% and amino acid similarity of 93% for the full length protein. In humans at least 3 variants have been predicted and they are all similar at the N-terminus. A number of expressed sequence tags (ESTs) cloned from murine undifferentiated ES cells and Lin-/c-Kit+/Sca-1+ hematopoietic stem cell cDNA libraries correspond to the Pogz gene, further underlining an important function for this gene in stem cells. In mice, deletion of the gene in all tissues early in embryogenesis has been shown to be lethal.