NFYA Rabbit Polyclonal Antibody

CAT#: TA319426

Rabbit polyclonal anti-NF-Y antibody

Product Images

WB of Rabbit Anti-NF-Y (A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:10,000, WB: 1:1,000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear transcription factor Y subunit alpha
• Database Link:
  NP_002496
  Entrez Gene 4800 Human
  UniProt ID P23511
• Synonyms: CBF-A; CBF-B; HAP2; NF-YA
• Note: Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research.

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation