Lefty2 Mouse Monoclonal Antibody [Clone ID: 7C5G1H6H10]

CAT#: TA319606

Mouse monoclonal LEFTY A antibody

Product Images

Mab anti-Human LEFTY antibody to detect by WB partially purified recombinant 6X His tagged human LEFTY. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY.

Specifications

Product Data

• Clone Name: 7C5G1H6H10
• Applications: WB
• Recommended Dilution: ELISA: 1:5,000, WB: 1:2,000
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: left-right determination factor 2
• Database Link:
  NP_796073
  Entrez Gene 7044 Human
  UniProt ID P57785
• Synonyms: EBAF; LEFTA; LEFTYA; MGC46222; TGF-beta-4; TGFB4
• Note: During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.