KEAP1 Rabbit Polyclonal Antibody

CAT#: TA319790

Rabbit Polyclonal KEAP1 Antibody

Product Images

Western blot analysis of KEAP1 in human lung tissue lysate with KEAP1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunofluorescence of KEAP1 in human lung tissue with KEAP1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1.
• Formulation: KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: KEAP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69 kDa
• Gene Name: kelch like ECH associated protein 1
• Database Link:
  NP_036421
  Entrez Gene 50868 MouseEntrez Gene 117519 RatEntrez Gene 9817 Human
  UniProt ID Q14145
• Background: KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.
• Synonyms: INrf2; KLHL19

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis