ORAI3 Mouse Monoclonal Antibody [Clone ID: 1B4F1]

CAT#: TA320076

Mouse ORAI3 Monoclonal Antibody

Product Images

Western blot analysis of ORAI3 in rat lung tissue lysate with ORAI3 antibody at 2 ug/mL.

Specifications

Product Data

• Clone Name: 1B4F1
• Applications: WB
• Recommended Dilution: WB: 2 ug/mL
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.
• Formulation: ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ORAI calcium release-activated calcium modulator 3
• Database Link:
  NP_689501
  Entrez Gene 309000 RatEntrez Gene 93129 Human
  UniProt ID Q9BRQ5
• Background: ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.
• Synonyms: TMEM142C

Reference Data

• Protein Families: Transmembrane