TBX21 Mouse Monoclonal Antibody [Clone ID: eBio39D (39D, 3-9D)]

CAT#: TA320469

Mouse Anti-T-bet Purified (25 ug)

Product Images

CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.

Specifications

Product Data

• Clone Name: eBio39D (39D, 3-9D)
• Applications: WB
• Recommended Dilution: IP, WB
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: T-box 21
• Database Link:
  NP_037483
  Entrez Gene 57765 MouseEntrez Gene 303496 RatEntrez Gene 30009 Human
  UniProt ID Q9UL17
• Background: The eBio39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-γ, and T-Bet also regulates the expression of IFN-γ, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-γ, T-Bet is also known to regulate the expression of IL-12Rβ and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
• Synonyms: T-bet; T-PET; TBET; TBLYM

Reference Data

• Protein Families: Druggable Genome, Transcription Factors