GATA1 Rabbit Polyclonal Antibody

CAT#: TA321429

Anti-GATA1 (Phospho-Ser142) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 43 kDa. Positive control: HL60 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: HL60 cells untreated with PMA lysate Lane 2: HL60 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using GATA1 (Phospho-Ser142) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

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  Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using GATA1 (Phospho-Ser142) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: GATA binding protein 1
• Database Link:
  NP_002040
  Entrez Gene 2623 Human
  UniProt ID P15976
• Background: GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
• Synonyms: ERYF1; GATA-1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors