HDAC4 Rabbit Polyclonal Antibody

CAT#: TA322533

Anti-HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 124; 140 kDa. Positive control: 293 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: 293 cells untreated with EGF lysate Lane 2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: 200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: histone deacetylase 4
• Database Link:
  NP_006028
  Entrez Gene 9759 Human
  UniProt ID P56524
• Background: Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
• Synonyms: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDACA

Reference Data

• Protein Families: Druggable Genome, Transcription Factors