BAG3 Rabbit Polyclonal Antibody

CAT#: TA322772

Anti-BAG3 rabbit polyclonal antibody

Product Images

Predicted band size: 62kDa. Positive control: Mouse muscle tissue and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane 1: Mouse muscle tissue lysate Lane 2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds)

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  Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using BAG3 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1000-2000, WB: 1:500-2000,IHC: 1:15-50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 200 amino acids of human BCL2-associated athanogene 3
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62 kDa
• Gene Name: BCL2 associated athanogene 3
• Database Link:
  NP_004272
  Entrez Gene 29810 MouseEntrez Gene 293524 RatEntrez Gene 9531 Human
  UniProt ID O95817
• Background: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
• Synonyms: BAG-3; BIS; CAIR-1; MFM6

Reference Data

• Protein Families: Druggable Genome