TRIM32 Rabbit Polyclonal Antibody

CAT#: TA323256

Anti-TRIM32 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 72 kDa. Positive control: Jurkat, PC3 and Lovo cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane 1: Jurkat cell lysate Lane 2: PC3 cell lysate Lane 3: Lovo cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)

• 

  Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using TRIM32 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2000-5000, WB: 1:500-2000,IHC: 1:50-200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 300 amino acids of human tripartite motif containing 32
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: tripartite motif containing 32
• Database Link:
  NP_001093149
  Entrez Gene 69807 MouseEntrez Gene 313264 RatEntrez Gene 22954 Human
  UniProt ID Q13049
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains; a RING; a B-box type 1 and a B-box type 2; and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus; where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
• Synonyms: BBS11; HT2A; LGMD2H; TATIP

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis