SPDYA Rabbit Polyclonal Antibody

CAT#: TA323468

Anti-SPDYA Rabbit Polyclonal Antibody

Product Images

Predicted band size: 36 kDa. Positive control: NT2D1, PC3 and U-67 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane 1: NT2D1 cell lysate Lane 2: PC3 cell lysate Lane 3: U-67 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36 kDa
• Gene Name: speedy/RINGO cell cycle regulator family member A
• Database Link:
  NP_001008779
  Entrez Gene 70891 MouseEntrez Gene 192209 RatEntrez Gene 245711 Human
  UniProt ID Q5MJ70
• Background: Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1,400 genes and comprises nearly 8% of the human genome.
• Synonyms: RINGO3; RINGOA; SPDY1; SPY1

Reference Data

• Protein Pathways: Oocyte meiosis, Progesterone-mediated oocyte maturation