HIP2 (UBE2K) Rabbit Polyclonal Antibody

CAT#: TA323779

Anti-UBE2K Rabbit Polyclonal Antibody

Product Images

Predicted band size: 22 kDa. Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22 kDa
• Gene Name: ubiquitin conjugating enzyme E2 K
• Database Link:
  NP_001104582
  Entrez Gene 53323 MouseEntrez Gene 289623 RatEntrez Gene 3093 Human
  UniProt ID P61086
• Background: Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ?-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3. E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin. E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-?, huntingtin, and MHC-heavy chain proteins. Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity.
• Synonyms: E2-25K; HIP2; HYPG; LIG; UBC1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis