EZH2 Mouse Monoclonal Antibody [Clone ID: 144CT2.1.1.5]

CAT#: TA324333

Mouse monoclonal EZH2 Antibody

Product Images

Western blot analysis of EZH2 antibody (Cat. #TA324333) in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.

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  EZH2 Monoclonal Antibody (Cat. #TA324333) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of U251 cell stained with EZH2 Antibody (Cat#TA324333/SG100830AA).U251 cells were incubated with EZH2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).EZH2 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Clone Name: 144CT2.1.1.5
• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:100~500, IHC: 1:10~50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1/IgK
• Clonality: Monoclonal
• Immunogen: This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85363 Da
• Gene Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
• Database Link:
  NP_694543
  Entrez Gene 709075 MonkeyEntrez Gene 2146 Human
  UniProt ID Q15910
• Synonyms: ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors