TDP43 (TARDBP) Rabbit Polyclonal Antibody

CAT#: TA324524

Rabbit polyclonal TARDBP Antibody (N-term)

Product Images

TARDBP Antibody (N-term) (Cat. #TA324524) western blot analysis in Hela cell line and mouse brain lysates (35ug/lane).This demonstrates the TARDBP antibody detected the TARDBP protein (arrow).

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  TARDBP Antibody (N-term) (Cat. #TA324524)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARDBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of Hela cell stained with TARDBP Antibody (N-term) (Cat#TA324524).Hela cells were incubated with TARDBP primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44740 Da
• Gene Name: TAR DNA binding protein
• Database Link:
  NP_031401
  Entrez Gene 230908 MouseEntrez Gene 23435 Human
  UniProt ID Q13148
• Synonyms: ALS10; TDP-43

Reference Data

• Protein Families: Transcription Factors