ULK1 Rabbit Polyclonal Antibody

CAT#: TA325024

Rabbit polyclonal APG1 (ULK1) Antibody (Center)

Product Images

Western blot analysis of anti-ULK1 Pab (Cat. #TA325024) in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

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  IF image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine, then incubated with TA325024 APG1 (ULK1) (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This APG1 (ULK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 642-672 amino acids from the Central region of human APG1 (ULK1).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 112631 Da
• Gene Name: unc-51 like autophagy activating kinase 1
• Database Link:
  NP_003556
  Entrez Gene 8408 Human
  UniProt ID O75385
• Synonyms: ATG1; ATG1A; hATG1; UNC51; Unc51.1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy