HMGA1 Rabbit Polyclonal Antibody

CAT#: TA325094

Rabbit polyclonal HMGA1 Antibody (C-term)

Product Images

HMGA1 Antibody (C-term) (Cat. #TA325094) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).

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  IF image of A549 cell stained with HMGA1 Antibody (C-term) (Cat#TA325094).A549 cells were incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).HMGA1 immunoreactivity is localized to Nucleus significantly.

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  HMGA1 Antibody (C-term) (Cat. #TA325094) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11676 Da
• Gene Name: high mobility group AT-hook 1
• Database Link:
  NP_002122
  Entrez Gene 15361 MouseEntrez Gene 117062 RatEntrez Gene 3159 Human
  UniProt ID P17096
• Synonyms: HMG-R; HMGA1A; HMGIY

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors