LXR alpha (NR1H3) Rabbit Polyclonal Antibody

CAT#: TA325122

Rabbit polyclonal NR1H3 Antibody (Center)

Product Images

Western blot analysis of NR1H3 Antibody (Center) (Cat. #TA325122) in MCF-7 cell line lysates (35ug/lane). NR1H3 (arrow) was detected using the purified Pab.

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  IF image of U251 cell stained with NR1H3 Antibody (Center) (Cat#TA325122).U251 cells were incubated with NR1H3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).NR1H3 immunoreactivity is localized to Nucleus significantly.

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  NR1H3 Antibody (Center) (Cat. #TA325122) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This NR1H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the Central region of human NR1H3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50396 Da
• Gene Name: nuclear receptor subfamily 1 group H member 3
• Database Link:
  NP_005684
  Entrez Gene 10062 Human
  UniProt ID Q13133
• Synonyms: LXR-a; LXRA; RLD-1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: PPAR signaling pathway