POLR2A Rabbit Polyclonal Antibody

CAT#: TA326076

Rabbit polyclonal POLR2A (Ab-1619) antibody

Product Images

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody (#TA326076, Line 1 and 2).The lane on the right is treated with the synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody (#TA326076).The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (Ab-1619) antibody (#TA326076).The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:3000 IHC: 1:50~1:100IF: 1:100~1:500 ELISA: 1:10000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-S-P-S).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: polymerase (RNA) II subunit A
• Database Link:
  NP_000928
  Entrez Gene 20020 MouseEntrez Gene 363633 RatEntrez Gene 5430 Human
  UniProt ID P24928
• Synonyms: hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Reference Data

• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase