CDK4 Rabbit Polyclonal Antibody

CAT#: TA326832

Rabbit anti-CDK4 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CDK4 antibody.

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using CDK4 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded human esophagus using CDK4 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat lung using CDK4 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded mouse lung using CDK4 antibody at dilution of 1:100 (400x lens).

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Center-peptide of human CDK4
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin-dependent kinase 4
• Database Link:
  NP_000066
  Entrez Gene 12567 MouseEntrez Gene 94201 RatEntrez Gene 1019 Human
  UniProt ID P11802
• Background: Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
• Synonyms: CMM3; PSK-J3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction