MTAP Rabbit Polyclonal Antibody

CAT#: TA326900

MTAP Rabbit Polyclonal Antibody

Product Images

Western blot analysis of HT-29 cell and liver cell lysate using MTAP antibody.

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  Immunofluorescence analysis of HeLa cell using MTAP antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MTAP
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: methylthioadenosine phosphorylase
• Database Link:
  NP_002442
  Entrez Gene 66902 MouseEntrez Gene 298227 RatEntrez Gene 4507 Human
  UniProt ID Q13126
• Background: MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5¡¯-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine. MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor .
• Synonyms: BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways