Ferritin Heavy Chain (FTH1) Rabbit Polyclonal Antibody

CAT#: TA326948

Rabbit anti-FTH1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using FTH1 antibody.

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  Immunohistochemistry of paraffin-embedded rat lung using FTH1 antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of MCF-7 cell using FTH1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human FTH1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ferritin heavy chain 1
• Database Link:
  NP_002023
  Entrez Gene 14319 MouseEntrez Gene 25319 RatEntrez Gene 2495 Human
  UniProt ID P02794
• Background: Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form. The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders. Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases.
• Synonyms: FHC; FTH; FTHL6; HFE5; PIG15; PLIF

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Porphyrin and chlorophyll metabolism