RHEB Rabbit Polyclonal Antibody

CAT#: TA326964

RHEB Rabbit Polyclonal Antibody

Product Images

Western blot analysis of brian cell and Hela cell lysate using RHEB antibody.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB: 1:500-1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human RHEB
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Ras homolog enriched in brain
• Database Link:
  NP_005605
  Entrez Gene 19744 MouseEntrez Gene 26954 RatEntrez Gene 6009 Human
  UniProt ID Q15382
• Background: Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberins GAP activity.
• Synonyms: RHEB2

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway