SF2 (SRSF1) Rabbit Polyclonal Antibody

CAT#: TA327123

Rabbit anti-SFRS1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using SRSF1/SF2/ASF antibody (TA327123) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SFRS1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: serine/arginine-rich splicing factor 1
• Database Link:
  NP_008855
  Entrez Gene 110809 MouseEntrez Gene 689890 RatEntrez Gene 6426 Human
  UniProt ID Q07955
• Background: SF2/ASF is a member of the Ser-Arg-rich (SR) protein family of highly conserved nuclear phosphoproteins involved in pre-mRNA splicing. Besides its role in nuclear pre-mRNA splicing, SF2/ASF has been shown to shuttle between the nucleus and cytoplasm, suggesting additional roles in mRNA transport and cytoplasmic events. SF2/ASF associates with translating ribosomes and stimulates translation. It also activates translation initiation by suppressing the activity of 4E-BP1, which is mediated by SF2/ASF association with mTOR and the phosphatase PP2A. More recent studies have demonstrated a role for SF2/ASF in microRNA processing.
• Synonyms: ASF; SF2; SF2p33; SFRS1; SRp30a

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome