UBE2I Rabbit Polyclonal Antibody

CAT#: TA327329

UBE2I Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using UBE2I antibody.

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  Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of U2OS cell using UBE2I antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human UBE2I
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin conjugating enzyme E2 I
• Database Link:
  NP_003336
  Entrez Gene 22196 MouseEntrez Gene 25573 RatEntrez Gene 7329 Human
  UniProt ID P63279
• Background: The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain. Localization of Ubc9 to the nucleus and the nuclear envelope allows this enzyme to catalyze target protein sumoylation and regulate target protein nucleocytoplasmic transport and transcriptional activity . Ubc9 target proteins include a host of proteins (RAD51, RAD52, p53 and c-Jun) that regulate the cell cycle, DNA repair, and p53-dependent processes.
• Synonyms: C358B7.1; P18; UBC9

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis