NONO Rabbit Polyclonal Antibody

CAT#: TA327576

Rabbit anti-Nono Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Nono antibody.

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  Immunohistochemistry of paraffin-embedded rat liver using NONO antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded rat brain using NONO antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded human colon using NONO antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded human esophageal using NONO antibody at dilution of 1:100 (x40 lens).

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  Immunohistochemistry of paraffin-embedded human gastric cancer using NONO antibody at dilution of 1:100 (x40 lens).

• Immunohistochemistry of paraffin-embedded mouse lung using NONO antibody at dilution of 1:100 (x40 lens).

• Immunohistochemistry of paraffin-embedded mouse brain using NONO antibody at dilution of 1:100 (x40 lens).

• Immunohistochemistry of paraffin-embedded mouse heart using NONO antibody at dilution of 1:100 (x40 lens).

• Immunofluorescence analysis of U20S cell using NONO antibody. Blue: DAPI for nuclear staining.

• Immunofluorescence analysis of U2OS cell using NONO antibody.

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A specific peptide of human Nono
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: non-POU domain containing, octamer-binding
• Database Link:
  NP_031389
  Entrez Gene 53610 MouseEntrez Gene 317259 RatEntrez Gene 4841 Human
  UniProt ID Q15233
• Background: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114

Reference Data

• Protein Families: Druggable Genome, Transcription Factors