P2Y1 (P2RY1) Rabbit Polyclonal Antibody

CAT#: TA328626

Rabbit Polyclonal Anti-P2Y1 Receptor (extracellular)

Product Images

Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates: 1-3. Anti-P2Y1 Receptor (extracellular) antibody, (1:200). 4-6. Anti-P2Y1 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  Indirect flow cytometry analysis of Jurkat (human acute T cell leukemia) living cells: black line, Unstained cells.green line, Cells stained + Anti-P2Y1 Receptor (extracellular) antibody, (5 ug/5x105 cells).

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  Expression of P2Y1 receptor in rat PC12 cells. Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) antibody (TA328626), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of extracellular staining with live view of the cells.

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  Indirect flow cytometry analysis of Jurkat (human acute T cell leukemia) living cells: black line: Unstained cells. Green line: Cells stained + Anti-P2Y1 Receptor (extracellular) antibody, (5 ug/5x105 cells)

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; FC: 1:50-1:600
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2Y1 Receptor. 2nd extracellular loop.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: purinergic receptor P2Y1
• Database Link:
  NP_002554
  Entrez Gene 25265 RatEntrez Gene 5028 Human
  UniProt ID P47900
• Background: The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation. The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14. P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.
• Synonyms: P2Y1
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction