Clcn1 Rabbit Polyclonal Antibody

CAT#: TA328782

Rabbit Polyclonal Anti-CLC-1

Product Images

Western blot analysis of rat skeletal muscle membranes: 1. Anti-CLC-1 antibody, (1:200). 2. Anti-CLC-1 antibody, preincubated with the control peptide antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CVHRLGRVLRRKLGED, corresponding to amino acid residues 102-117 of rat CLC-1. Cytoplasmic, N-terminal part.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: chloride voltage-gated channel 1
• Database Link:
  NP_037279
  Entrez Gene 25688 Rat
  UniProt ID P35524
• Background: CLC-1 is a member of the voltage-dependent Cl channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7. CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl/H+ antiporters. The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. The ClC-1 channel is almost exclusively expressed in skeletal muscle where it has a dominant role in maintaining the membrane potential at rest and is important for repolarization of the skeletal muscle cells. Mutations in the ClC-1 gene result in myotonia congenita, a condition in which an increase in the excitability of skeletal muscle leads to repetitive action potentials, stiffness, and delayed relaxation. The disease can be either autosomal dominant (Thomsen's disease), or recessive (Becker's myotonia).
• Synonyms: ClC-1; CLC1; MGC138361; MGC142055