Gabrg2 Rabbit Polyclonal Antibody

CAT#: TA328812

Rabbit Polyclonal Anti-GABA (A) gamma2 Receptor (extracellular)

Product Images

Western blot analysis of rat brain membranes: 1. Anti-GABA(A) ?2 Receptor (extracellular) antibody, (1:200). 2. Anti-GABA(A) ?2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

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  ICC staining of rat living hippocampal neurons. A. Extracellular staining of cells using Anti-GABA(A)gamma 2 Receptor (extracellular) antibody, (1:100). Representative images from time course experiments with cultured neurons undergoing TNF-a treatment ranging from 10 min to 6 h in duration.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide QKSDDDYEDYASNKT(C), corresponding to amino acid residues 39-53 of rat GABA(A) ?2 Receptor. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type A receptor gamma 2 subunit
• Database Link:
  NP_899156
  Entrez Gene 2566 HumanEntrez Gene 14406 MouseEntrez Gene 29709 Rat
  UniProt ID P18508
• Background: The neurotransmitter GABA (?-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors.GABAA R belongs to the ligand gated ion channel superfamily.It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: a, Ã?, ?, d, e, p, ?, and ?.The native GABAA receptor, in most cases, consists of 2a, 2Ã? and 1? subunit. Three ? subunits genes have been identified in mammals. The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.Recently, a genetic linkage between familial epilepsy syndrome and mutations in the ?2 subunit of the GABAA receptor have been demonstrated.
• Synonyms: CAE2; ECA2; GEFSP3
• Note: This antibody was tested in live cell imaging. Please see IF/ICC data for detail.