Kcnj5 Rabbit Polyclonal Antibody

CAT#: TA328939

Rabbit polyclonal Anti-Kir3.4 (GIRK4)

Product Images

Western blot analysis of rat heart membranes: 1. Anti-Kir3.4 (GIRK4) antibody, (1:200). 2. Anti-Kir3.4 (GIRK4) antibody, preincubated with the control antigen.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4 .? ? Intracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: potassium voltage-gated channel subfamily J member 5
• Database Link:
  NP_058993
  Entrez Gene 3762 HumanEntrez Gene 16521 MouseEntrez Gene 29713 Rat
  UniProt ID P48548
• Background: Kir3.4 is a member of the G-protein regulated inward-rectifier K+ (GIRK) channel subfamily which is part of an inward-rectifier K+ channel superfamily. The GIRK subfamily comprises four members in mammals (Kir3.1- Kir3.4) that present the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly.Kir3.4 and the other Kir3 family subunits can be activated by neurotransmitters and other factors via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of G a-GTP from the GÃ?? dimer. The latter directly binds to Kir3 and activates the channel. Kir3.4 expression is largely confined to the heart, where it co-assembles with Kir3.1 to form the prototypical muscarinic-gated K+ channel KACh. Indeed, knockout mice for Kir3.4 showed impaired heart rate following vagal nerve stimulation.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin was shown to be a potent blocker of Kir3.4 containing channels (8.6 nM for the Kir3.1/3.4 combination).4
• Synonyms: CIR; GIRK4; KATP-1; KATP1; KIR3.4