EEA1 Rabbit Polyclonal Antibody

CAT#: TA329101

Rabbit Polyclonal anti-EEA1 antibody

Product Images

WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta

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  Rabbit Anti-EEA1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution; Primary Antibody Concentration: 1:100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1:200; Magnification: 20X; Exposure Time: 0.5 � 2.0 sec; Protocol located in Reviews and Data.

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  Amount and Sample Type : 500 ug rat brain homogenate; Amount of IP Antibody : 6 ug; Primary Antibody : EEA1; Primary Antibody Dilution : 1:500; Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution : 1:5000; Gene Name : EEA1; Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: WB, IP
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-EEA1 antibody: synthetic peptide directed towards the N terminal of human EEA1. Synthetic peptide located within the following region: LTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSAS
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 162 kDa
• Gene Name: early endosome antigen 1
• Database Link:
  NP_003557
  Entrez Gene 314764 RatEntrez Gene 8411 Human
  UniProt ID Q15075
• Background: EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
• Synonyms: MST105; MSTP105; ZFYVE2
• Note: Immunogen sequence homology: Human: 100%; Pig: 92%; Rat: 92%; Horse: 92%; Mouse: 92%; Bovine: 92%; Guinea pig: 92%; Rabbit: 85%; Dog: 75%

Reference Data

• Protein Pathways: Endocytosis