14-3-3 beta (YWHAB) Rabbit Polyclonal Antibody

CAT#: TA332392

Rabbit anti-YWHAB Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using YWHAB antibody.

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  Immunohistochemistry of paraffin-embedded human kidney using YWHAB antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of HeLa cell using YWHAB antibody.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human YWHAB
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 246
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• Database Link:
  NP_647539
  Entrez Gene 54401 MouseEntrez Gene 56011 RatEntrez Gene 7529 Human
  UniProt ID P31946
• Background: 3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, Ï?, ζ, Ï?, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
• Synonyms: GW128; HEL-S-1; HS1; KCIP-1; YWHAA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis