Cardiac Troponin I (TNNC1) Rabbit Polyclonal Antibody

CAT#: TA332463

Rabbit anti-TNNC1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TNNC1 antibody.

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  Immunofluorescence analysis of U2OS cell using TNNC1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500 - 1:2000, IHC 1:50- 1:200, IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human TNNC1
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 161
• Gene Name: troponin C1, slow skeletal and cardiac type
• Database Link:
  NP_003271
  Entrez Gene 21924 MouseEntrez Gene 290561 RatEntrez Gene 7134 Human
  UniProt ID P63316
• Background: Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).
• Synonyms: CMD1Z; CMH13; TN-C; TNC; TNNC

Reference Data

• Protein Pathways: Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)