U2AF65 (U2AF2) Rabbit Polyclonal Antibody

CAT#: TA332467

Rabbit anti-U2AF2 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using U2AF2 antibody.

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  Immunofluorescence analysis of U2OS cell using U2AF2 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:100
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54kDa
• Gene Name: U2 small nuclear RNA auxiliary factor 2
• Database Link:
  NP_009210
  Entrez Gene 22185 MouseEntrez Gene 11338 Human
  UniProt ID P26368
• Background: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
• Synonyms: U2AF65

Reference Data

• Protein Pathways: Spliceosome