DDX3 (DDX3X) Rabbit Polyclonal Antibody

CAT#: TA332805

Rabbit anti-DDX3X Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DDX3X antibody.

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  Immunohistochemistry of paraffin-embedded human breast cancer using DDX3X antibody at dilution of 1:200 (400x lens).

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  Immunofluorescence analysis of U20S cell using DDX3X antibody. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cell using DDX3X antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200;IF 1:50 - 1:200;IP 1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human DDX3X
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73 kDa
• Gene Name: DEAD-box helicase 3, X-linked
• Database Link:
  NP_001347
  Entrez Gene 13205 MouseEntrez Gene 317335 RatEntrez Gene 1654 Human
  UniProt ID O00571
• Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing results in multiple transcript variants.
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway