DDX3 (DDX3X) Rabbit Polyclonal Antibody

CAT#: TA332805

Rabbit anti-DDX3X Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DDX3X antibody.

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  Immunofluorescence analysis of U20S cell using DDX3X antibody. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cell using DDX3X antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC-P,1:50 - 1:200
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 300μg-600μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73kDa
• Gene Name: DEAD-box helicase 3, X-linked
• Database Link:
  NP_001347
  Entrez Gene 13205 MouseEntrez Gene 317335 RatEntrez Gene 1654 Human
  UniProt ID O00571
• Background: The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway