TLR6 Mouse Monoclonal Antibody [Clone ID: 86B1153.2]

CAT#: TA336394

Mouse Monoclonal TLR6 Antibody (86B1153.2)

Product Images

Flow (Intracellular): TLR6 Antibody (86B1153.2) TA336394 - Analysis using the PE conjugate of TA336394. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.

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  Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) TA336394 - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Flow Cytometry: TLR6 Antibody (86B1153.2) TA336394 - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used.

Specifications

Product Data

• Clone Name: 86B1153.2
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry: 1:10-1:500, Flow Cytometry: 3 ug/10-6 cells, Flow (Intracellular), Flow (Cell Surface), Western Blot
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 6
• Database Link:
  NP_006059
  Entrez Gene 10333 Human
  UniProt ID Q9Y2C9
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa. Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6. TLR6 is predominantly expressed in spleen, thymus, ovary, and lung.
• Synonyms: CD286
• Note: Immunohistochemistry-Paraffin and Flow cytometry/(Intracellular)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway