D4 (ARHGDIB) Mouse Monoclonal Antibody [Clone ID: 10D774]

CAT#: TA336402

Mouse Monoclonal D4-GDI/RhoGDI2 Antibody (10D774)

Product Images

Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) TA336402 - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or TA336402 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: TA336402 which recognizes both full-length and cleaved D4-GDI.

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  Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) TA336402 - Western blot analysis of TA336402 in whole Jurkat cell lysate.

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  Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) TA336402 - Analysis of D4GDI in Jurkat cells using TA336402 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. TA336402 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.

Specifications

Product Data

• Clone Name: 10D774
• Applications: FC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Flow Cytometry, Western Blot: 1-2 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Rho GDP dissociation inhibitor beta
• Database Link:
  NP_001166
  Entrez Gene 397 Human
  UniProt ID P52566
• Background: D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
• Synonyms: D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway