Caspase 3 (CASP3) Mouse Monoclonal Antibody [Clone ID: 31A893]

CAT#: TA336456

Mouse Monoclonal Caspase-3 Antibody (31A893)


USD 424.00

5 Days*

Size
    • 100 ug

Frequently bought together (3)
Recombinant protein of human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
    • 20 ug

USD 823.00


Transient overexpression lysate of caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
    • 100 ug

USD 396.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 159.00

Other products for "Caspase 3"

Specifications

Product Data
Clone Name 31A893
Applications IHC, WB
Recommended Dilution Immunocytochemistry/ Immunofluorescence, Western Blot: 2 ug/ml, Immunohistochemistry: 5 ug/mL, Immunohistochemistry-Paraffin: 5 ug/ml
Reactivities Human
Host Mouse
Isotype IgG
Clonality Monoclonal
Immunogen A recombinant full-length human Caspase-3 protein was used as the immunogen for this antibody.
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name caspase 3
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family and is a downstream effector cysteine protease in the apoptotic pathway. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. The protein is ubiquitously expressed in normal human tissues including the liver, spleen, heart, liver and kidney.
Synonyms CPP32; CPP32B; SCA-1
Note For IHC-P validation of this target, 1M EDTA pH 9.0 buffer was used (citrate buffer ph 6.0 did not work)
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protease
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.