D4 (ARHGDIB) Mouse Monoclonal Antibody [Clone ID: 97A1015]

CAT#: TA336465

Mouse Monoclonal D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015)

Product Images

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - Analysis using the FITC conjugate of TA336465. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated (right) Ramos cells using 0.5 ug of was used for this test.

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  Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - Analysis using the FITC conjugate of TA336465. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells using 0.5 ug of NB100-56060. The shaded histogram represents cells without antibody.

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  Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (TA336465, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.

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  Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.

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  Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug antibody/test/1 million cells) and ((as seen in B). this is because (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.

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  Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and TA336465 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (TA336465) IMG-160A specifically detects only the cleaved form of the protein.

• Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) TA336465 - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.

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Specifications

Product Data

• Clone Name: 97A1015
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 ug/10^6 cells, Immunohistochemistry: 1:10-1:500, Immunoprecipitation: 1-2 ug/ml, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin: 1:10-1:500, Flow (Intracellular): 0.5-1 ug/10^6 cells
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Rho GDP dissociation inhibitor beta
• Database Link:
  NP_001166
  Entrez Gene 11857 MouseEntrez Gene 397 Human
  UniProt ID P52566
• Background: D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
• Synonyms: D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway