FTO Rabbit Polyclonal Antibody

CAT#: TA336541

Rabbit Polyclonal FTO Antibody

Product Images

Western Blot: FTO Antibody TA336541 - Detection of FTO in human brain lysate using anti-FTO TA336541.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunoblotting, Immunohistochemistry, Western Blot: 0.5 ug/ml
• Reactivities: Human, Mouse, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1]
• Formulation: PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60 kDa
• Gene Name: fat mass and obesity associated
• Database Link:
  NP_001073901
  Entrez Gene 26383 MouseEntrez Gene 79068 Human
  UniProt ID Q9C0B1
• Background: FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).
• Synonyms: ALKBH9; BMIQ14; GDFD
• Note: This FTO antibody is useful for Western blot analysis, where a band is seen at ~60 kDa.